Organoid Single Cell Atlas
OSCA to Shed light on Concealed Actuality
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COVID-19
SARS-CoV-2, the virus responsible for COVID-19, causes acute respiratory distress syndrome (ARDS) by damaging lung epithelial cells. Here we use air-liquid interface culture (ALI-culture) to make fully differentiated lung airway cells from organoids. We infect SARS-CoV-2, MERS-CoV, or Influenza-A viruses on organoids to compare response. This data contains 200k cells from 20 organoids.
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LUNG
Lung airway epithelium contains many cells; Basal, Club, Goblet, Ciliated, Ionocyte, Brush, Pulmonary neuroendocrine cells. The high proportion of immune and stromal cells make it difficult to study the epithelial cells of the lung in depth. Here we show many epithelial cells from a tissue, 3d cultured organoid, and air-liquid interface cultured (ALI-culture) organoids. It is also possible to analyze rare cells (Ionocyte, Brush cell, PNEC, etc.) in the lungs through the largest single-cell RNA database. This data contains 300k cells from a tissue and 30 organoids.
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GASTRIC
The gastric epithelium is the lining of the stomach that serves as a protective barrier against gastric acid and other harmful substances. It harbors both constitutively active progenitor populations and differentiated cell populations that can be recruited as stem cells. Culturing these stem cells can generate gastric organoids. The gastric epithelium consists of gastric pit cells, chief cells, parietal cells, enterochromaffin-like cells, and stem cells. OSCA's gastric organoid atlas provides a comprehensive catalog of gene expression patterns and cell types within the organoid, offering insights into the cellular composition and organization of the stomach. This data contains 30K cells from 18 organoids.
ABOUT
Single-cell RNA sequencing technology makes it possible to analyze by single-cell level. Human adult stem cell-derived organoids recapitulate the epithelial heterogeneity of each organ. Organoid scRNA-seq allows us to identify the characteristics of various cell types. Because organoids have only epithelial cells, they are suitable for research such as tissue regeneration, carcinogenesis, or organ development.
Organoids are highly reproducible experimental models. Using organoids, we can continue to study response to drug treatment, viral infections, etc., rather than just profiling the characteristics of cells. Moreover, adult stem cell-derived organoids are in vitro models that best represent human physiology and pathology. Organoids are better in that they are cost-effective than animal models and can conduct human-specific research like ACE2+ cells in airway epithelium for SARS-CoV-2 infection.
